FIG 3.

Fluorescence-based mutation reporter recapitulates the mutation rate increase of an S. cerevisiae mutant. (A) The yEGFP-NAT cassette was integrated at the CAN1 locus of S. cerevisiae using homology-based targeting. (B) The assay detected the mutator phenotype of the shu1Δ mutant, previously shown to have an elevated CAN1 mutation rate (34). The assay also showed that spontaneous forward mutation in C. glabrata during standard laboratory growth in YPD is not higher than that in S. cerevisiae. Sc, S. cerevisiae; Cg, C. glabrata. Error bars, 95% confidence intervals.