FIG 3.

Replication profiles of wild-type, V142I Vif, or cloned RN18 analog-resistant (RAR) viruses in the absence or presence of IMC15 antagonist. Cells were infected with equivalent amounts of HIV-1LAI, and virus replication was monitored over time based on reverse transcriptase activity in culture supernatants. (a) Wild-type virus was sensitive to IMC15, while both the V142I Vif point mutant and RAR virus replicated efficiently in the presence of antagonist. (b) All viruses replicated similarly in permissive MT4 cells that did not express A3G. Data are representative of results from at least three independent experiments.