FIG 7.

Viruses harboring resistance mutations exhibit a fitness advantage over wild-type virus in cells expressing A3G. (a) Selective primers were designed to generate allele-specific qPCR assays to measure relative amounts of viruses. (b and c) The codon for valine 142 is shown in bold. Replication profiles were generated in nonpermissive (b) and permissive (c) cells infected with single virus stocks or equivalent amounts of wild-type and mutant virus stocks. (d and e) The relative percentages of viruses in dual infections of nonpermissive H9 cells (d and e) with valine 142 (V142) (d) and RAR (e) were determined by allele-specific quantitative PCR of cDNA generated from viral RNA isolated from culture supernatants over time. Data are representative of results from at least three independent experiments.