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. 2019 Feb 26;10(1):e02915-18. doi: 10.1128/mBio.02915-18

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Copyright © 2019 Grunenwald et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — S. aureus requires mntE for growth in the presence of excess Mn. (A) S. aureus strains streaked onto TSA plates supplemented with 0, 0.5, and 1.0 mM MnCl₂. (B) Growth as measured by OD₆₀₀ over time for S. aureus strains in TSB medium supplemented with 0, 0.5, or 1.0 mM MnCl₂. The data are the average of the means from three independent experiments each in biological triplicate with the standard error of the mean shown. (C) Growth as measured by OD₆₀₀ over time for S. aureus strains containing either an empty plasmid (pOS1 Plgt [p.P_lgt]) or a plasmid containing constitutively expressed mntE (pOS1 P_lgtmntE [p.P_lgtmntE]) in TSB supplemented with 1 mM MnCl₂. The data are the average of the means from three independent experiments, each in biological triplicate with the standard error of the mean shown.