FIG 5.

Inactivation of mntE leads to accumulation of intracellular Mn and a reduction in intracellular Fe levels. Concentrations of intracellular Mn (A), Fe (B), Zn (C), Co (D), Ni (E), and Cu (F) were measured using inductively coupled plasma mass spectrometry and normalized to intracellular sulfur for S. aureus strains grown under metal-replete (TSB) and metal-deplete (TSB plus 100 mM EDTA) conditions. The data are the average of the means from three independent experiments, each in biological triplicate with error bars representing standard error. *, P < 0.05, **, P < 0.01, and ****, P < 0.0001, calculated by two-way ANOVA with Dunnett’s correction for multiple comparisons, comparing metal levels for each strain with WT S. aureus. n.s., not significant.