Table 5.
IL-1ß/IL-10 ratios and Mitochondrial function in ASD cells and non-ASD control cells.
| IL-1ß/IL-10 ratioa based ASD cell subgroups | Non-ASD controls (N = 38) | Krushkal wallis test | |||
|---|---|---|---|---|---|
| High (N = 56)e | Normal (N = 59) | Low (N = 22) | |||
| IL-1ß/IL-10 RATIOS CULTURED WITH P-VALUE | |||||
| medium | 1.54 ± 2.03d | 0.79 ± 1.12 | 0.32 ± 0.30 | 0.88 ± 0.92 | < 0.00001 |
| LPSc | 2.03 ± 1.43 | 1.19 ± 0.51 | 0.77 ± 0.56 | 1.81 ± 2.02 | < 0.00001 |
| Zymosan | 5.86 ± 3.94 | 2.53 ± 0.87 | 1.42 ± 0.74 | 3.25 ± 1.98 | < 0.00001 |
| CL097 | 9.40 ± 16.84 | 2.89 ± 2.84 | 2.72 ± 2.19 | 3.95 ± 2.77 | 0.00061 |
| MITOCHONDRIAL RESPIRATIONb P-VALUE | |||||
| PLR | 6.2 ± 5.2 | 8.3 ± 5.2 | 7.5 ± 3.8 | 7.9 ± 5.8 | 0.06771 |
| ALR | 27.2 ± 10.8 | 31.2 ± 14.0 | 27.3 ± 15.7 | 27.7 ± 13.2 | 0.5153 |
| MRCf | 93.3 ± 57.2 | 104.7 ± 59.0 | 84.6 ± 59.3 | 70.8 ± 44.4 | 0.0269 |
| RC | 59.9 ± 49.5 | 64.6 ± 48.5 | 49.7 ± 47.9 | 35.1 ± 33.6 | 0.00788 |
IL-1ß/IL-10 ratios are those obtained from purified ASD monocytes cultured with medium only, LPS (0.1 μg/ml), zymosan (50 μg/ml), or C097 (20 μM) as detailed in the materials and methods section. IL-1ß/IL-10 ratios were calculated as IL-1ß levels/IL-10 levels in each culture condition. The high ratio group revealed higher ratios than normal and low ratio groups under all the culture conditions than the normal and low ratio groups (p < 0.005 by two tailed Mann-Whitney test). The normal ratio group also revealed higher ratios than the low ratio group under the culture condition tested (p < 0.005, by two tailed Mann-Whitney test), except for the cultures under CL097.
Mitochondrial respiration parameters were measured in PBMCs.
Abbreviations used; ALR, ATP-linked respiration; LPS, lipopolysaccharide; MRC, Maximum respiration capacity; PLR, proton-leak respiration; RC, reserve capacity.
All the data are expressed as a mean value ± SD.
In 10 ASD subjects, samples were obtained at 2 time points, and in 3 ASD subjects. samples were obtained at 3 time points.
MRC and RC were higher than non-ASD controls in the high (p < 0.05 for MRC, p < 0.02 for RC), and normal (p < 0.005 for both MRC and RC) ratio ASD subgroups by two tailed Mann Whitney test.