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. 2019 Jan 17;58(6):1616–1620. doi: 10.1002/anie.201805238

Figure 1.

Figure 1

Semisynthesis of Tau4 [CML294]. A) First NCL with TauM [CML294] (4 mm) and TauC (2 mm) for 48 h at 37 °C, monitored by SDS‐PAGE. Lane 1: reaction at t=0; 2: reaction at 24 h; 3: reaction at 48 h; 4: purified Tau291‐441 [CML294]. Mass spectra show the ligation product before and after removal of the thiol‐protecting group Acm. B) Second ligation reaction between Tau291–441 [CML294] and TauN at 2 mm concentration each for 4 h at 37 °C, monitored by SDS‐PAGE. Lane 1: reaction at t=0; 2: reaction at 4 h; 3: purified Tau4 [CML294]; 4: recombinant Tau4. HPLC and mass spectrum of the purified ligation product Tau4 [CML294] are shown on the right. For analytical data of all four Tau4 variants, see Figures S8–11 in the Supporting Information.