Figure 2.

Different modes of Min oscillations in GUVs. A–C) Pulsing oscillations: MinD oscillates between the inside of the vesicles and the inner membrane surface. Dotted lines in (A) indicate ROIs for kymograph and intensity plots. D–F) Pole‐to‐pole oscillations: A protein binds alternately to the membrane of the two hemispheres of the vesicle. A second type of pole‐to‐pole oscillation is shown in Figure S3. G–I) Circling waves: Traveling waves that continuously revolve on the inside surface of the GUV. J–L) Trigger waves: Traveling waves that originate and terminate on opposing poles; here the wave origin is on the left side of the vesicle. The top panels [(A), (D), (G), and (J)] show two frames from a time series of confocal images of each oscillation mode. The fluorescence signal of eGFP‐MinD is shown in cyan. Magenta arrows indicate the directions of the waves. Scale bars: 10 μm. The center panels [(B), (E), (H), and (K)] show kymographs along the circumference of each vesicle [indicated as a magenta dotted line in (A)(1)]. The bottom panels [(C), (F), (I), and (L)] show average intensities in certain ROIs for each vesicle. ROIs are indicated by the dotted circle and boxes in (A)(2) in the respective colors of the curves. Magenta: Intensity of a membrane section (normalized); blue: intensity of the lumen close to the membrane section; orange: average intensity of entire lumen. Movie S1 shows all oscillation modes sequentially.