Fig 1.

NSGS mice transplanted with normal cells co-cultured on FA-AML MSCs exhibit haematopoietic stem and progenitor cells and myeloid expansion. 5 × 10⁵ normal bone marrow (BM) cells co-cultured on mesenchymal stromal cells (MSCs) from healthy donors (HD), Fanconi anaemia (FA) patients with cytopenias but no cancer (FA-BMF) or FA patients with acute myeloid leukaemia (FA-AML) (3–5 BM samples for each group) were transplanted into sublethally-irradiated NSGS mice. Recipients were intraperitoneally (i.p.) injected with the GVHD inhibitor OKT3 (10 μg/kg) at 24 h and 1 week after transplant. BM cells were subjected to flow cytometry analysis for human cell content at 12 weeks post-transplant. (A) Representative flow cytometry plots of total human engraftment (hCD45⁺; left), CD34⁺ (middle) and myeloid (CD33⁺)/lymphoid (CD19⁺) cells. (B) Quantification of human cell content by flow cytometry analysis depicted in (A). Results are means ± SD of three independent experiments (n = 9–12 for each group). **P < 0·01. [Colour figure can be viewed at wileyonlinelibrary.com]