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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Invest Dermatol. 2018 Aug 16;139(1):146–156. doi: 10.1016/j.jid.2018.07.025

Figure 2. IL-1R signaling in γδ T cells is essential to induce skin inflammation.

Figure 2.

(a) Reconstituted WT or IL-1R KO chimeric mice (n=3) were treated daily for 5 days with IMQ or vehicle control. Representative H&E-stained sections and frozen sections stained with Gr-1 are shown. Gr-1 positive cells are brown. Skin tissues were also stained with CD45 and Gr-1 assessed by flow cytometry. Epidermal thickness and percentage of CD45+Gr-1+ cells were measured. Scale bar =100 μm. Data are representative of two independent experiments with similar results. (b) Reconstituted mice (n=3) with WT γδ T cells or IL-1R deficiency in γδ T cells were treated daily for 5 days with IMQ or vehicle control. Representative H&E-stained sections and frozen sections stained with Gr-1 are shown. Gr-1 positive cells are brown. Skin tissues were also stained with CD45 and Gr-1 assessed by flow cytometry. Epidermal thickness and percentage of CD45+Gr-1+ cells were measured. Scale bar =100 μm. Data are representative of two independent experiments with similar results. The mRNA levels of IL-17, IL-22, TNF-α and IL-6 were measured by real-time PCR analysis. *p< 0.05, **p< 0.01.