Figure 3.
Photo-triggered HA-APP hydrogels control macrophage-ECM adhesive interactions and αvβ3 integrin expression. (A) Immunofluorescent images of αvβ3 integrin expression in pre-polarized M0, M1 and M2 macrophages in unmodified 3-D HA hydrogels. Scale bar is 30 μm. (B) Immunofluorescent images of αvβ3 integrin expressions of M0 macrophages in 3-D HA-APP hydrogels with increasing one-time UV exposure. Scale bar is 30 μm. (C) Quantified αvβ3 integrin expression of inactivated M0 and pre-polarized M1 and M2 macrophages in 3-D HA-APP hydrogels with increasing one-time UV exposure. (D) Quantified αvβ3 integrin expression of M0 macrophages in 3-D HA-APP hydrogels with one-time and periodic UV exposure. (E) Quantified cell attachment of M0 macrophages on 2-D HA-APP hydrogel substrates in response to increasing one-time and periodic UV exposure. (F) Quantified cell spreading area of M0 macrophages on 2-D HA-APP hydrogel substrates in response to increasing one-time and periodic UV exposure. Error bars represent ± S.E.M. from 3 independent experiments. P-values were calculated using the Student’s t-test. *, P < 0.05.