REAGENT or RESOURCE | SOURCE | IDENTIFIER | ||
---|---|---|---|---|
Bacterial and Virus Strains | ||||
AAV5.hSyn.oChIEF.tdTomato | Lin et al., 2009; Nabavi et al., 2014 | Duke Viral Vector Core (via Roger Tsien): #268 | ||
AAV5.hSyn.tdTomato (Control) | Lin et al., 2009; Nabavi et al., 2014 | Duke Viral Vector Core Control | ||
Chemicals, Peptides, and Recombinant Proteins | ||||
Cocaine HCl | NIDA Drug Supply Program | 9041–001 | ||
0.9% Saline | Fisher Scientific | NC0291799 | ||
Ketamine HCl | Henry Schein | 055853 | ||
Xylazine | Butler Schein | 033198 | ||
Rimadyl | Henry Schein | 024751 | ||
Lactated Ringer’s | Henry Schein | 009846 | ||
Betadine | Butler Schein | 038250 | ||
Ethanol | University of Pittsburgh Chemistry Stockroom | 200C5000 | ||
Gentamicin | Henry Schein | 006913 | ||
Heparin | Henry Schein | 055737 | ||
N-methyl-D-glucamine | Sigma-Aldrich | M2004 | ||
Sodium chloride | Sigma-Aldrich | S7653 | ||
Potassium chloride | Sigma-Aldrich | P9333 | ||
Sodium phosphate | Sigma-Aldrich | S9638 | ||
Sodium bicarbonate | Sigma-Aldrich | S5761 | ||
HEPES | Sigma-Aldrich | H3375 | ||
D-Glucose | Sigma-Aldrich | G8270 | ||
Sodium L-Ascorbate | Sigma-Aldrich | A7631 | ||
Thiourea | Sigma-Aldrich | T8656 | ||
Sodium pyruvate | Sigma-Aldrich | P2256 | ||
Magnesium sulfate | Sigma-Aldrich | 203726 | ||
Calcium chloride | Fisher Scientific | C1016 | ||
Hydrochloric Acid | Fisher Scientific | 0219405490 | ||
Cesium methanesulfonate | Fisher Scientific | C1426 | ||
Cesium chloride | Fisher Scientific | 289329 | ||
EGTA | Fisher Scientific | E3889 | ||
TEA-Chloride | Fisher Scientific | T2265 | ||
ATP Magnesium Salt | Fisher Scientific | A9187 | ||
GTP Sodium Salt | Fisher Scientific | G8877 | ||
QX-314-Cl | Alomone Labs | Q-150 | ||
Sodium phosphocreatine | Fisher Scientific | P7936 | ||
L-glutathione | Fisher Scientific | G4251 | ||
Potassium methanesulfonate | Fisher Scientific | 83000 | ||
Cesium hydroxide | Fisher Scientific | 516988 | ||
Potassium hydroxide | Fisher Scientific | P5958 | ||
Isoflurane | Henry Schein | 029405 | ||
DMSO | Fisher Scientific | BP231–1 | ||
Picrotoxin | Fisher Scientific | AC131210010 | ||
Sodium Pentobarbital | Henry Schein | 024352 | ||
Paraformaldehyde | Sigma-Aldrich | P6148 | ||
Fluoroshield with DAPI | Sigma-Aldrich | F6057 | ||
Lidocaine | Butler Schein | 014583 | ||
Experimental Models: Organisms/Strains | ||||
R. norvegicus: Sprague Dawley | Envigo | https://www.envigo.com | ||
Order Code #002 | ||||
Software and Algorithms | ||||
MedPC IV | MedAssociates | http://www.medassociates.com | ||
Clampex | Molecular Devices | https://www.moleculardevices.com/ | ||
Clampfit 10.3 | Molecular Devices | https://www.moleculardevices.com/ | ||
GraphPad Prism 6 | GraphPad | https://www.graphpad.com/ | ||
Virtual Slide Fluorescence Software (VS-ASW FL) | Olympus | http://www.olympus-lifescience.com/en/ |