Time-scaled phylogeny of O89b-containing E. coli strains. Molecular phylogeny based on the HKY85 model was calculated using a BEAST2 MultiTypeTree module of 6,890 core SNVs from 39 Sanji-related complete genomes plus 19 ST167 assemblies isolated between 1999 and 2010, as well as MG1655. The tree shown was generated by using R package ggtree, with associated 7-gene MLST, isolation region, source, and in silico O-antigen and H-antigen serotypes indicated.