Figure 5.

Differentiated adipocytes and fat depots express CXCR2. (A) 3T3‐L1 cells were differentiated into adipocytes and cells stained with oil red‐O (scale bars: 50 μm). (B) mRNA was extracted before and after differentiation, reverse transcribed to cDNA and analyzed for CXCR2 expression relative to the house‐keeping gene 18s. (C) Oil red‐O staining was quantified in undifferentiated and differentiated adipocytes in the absence or presence of two different CXCR2 inhibitors (expressed relative to differentiated cells). (D) Adipocytes differentiated in the absence and presence of a CXCR2 inhibitor 1 were analyzed for PPARγ protein expression relative to GAPDH levels (left panel) and quantified relative to GAPDH using densitometry. Data are plotted as mean (±sem), where each symbol represents an experimental replicate. Analyzed using an unpaired t test with Welch's correction (B and D) or one‐way ANOVA with TUKEY's multiple comparison test (C). Data are representative of two separate experiments