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. 2019 Apr 8;8:70. Originally published 2019 Jan 18. [Version 2] doi: 10.12688/f1000research.17430.2

Figure 8. Effect of GA and quercetin on various traits of P. aeruginosa, in vitro.

Figure 8.

‘Control’ in this figure is the vehicle control (0.5%v/v DMSO), which did not exert any effect on growth and pigment production of P. aeruginosa; Bacterial growth was measured as OD 764; OD of pyoverdine was measured at 405 nm, and Pyoverdine Unit was calculated as the ratio OD 405/OD 764 (an indication of pyoverdine production per unit of growth), Pyocyanin Unit was calculated as the ratio OD 520/OD 764 (an indication of pyocyanin production per unit of growth); Crystal violet assay was performed to measure biofilm formation, and biofilm eradication, followed by the measurement of OD at 580 nm. ( A) Effect of GA on growth and QS-regulated pigment production in P. aeruginosa. ( B) Effect of quercetin on growth and QS-regulated pigment production in P. aeruginosa. * p<0.05, ** p<0.01, *** p<0.001; AS, antibiotic susceptibility; QS, quorum sensing; GA, gallic acid.