Table 1. Effect of PGPE on catalase and haemolytic activity of test bacteria.
Organism | Concentration of
extract, μg/ml |
% Change in catalase
activity (mean ± SD) |
% Inhibition in
Hemolysis (Mean ± SD) |
---|---|---|---|
C. violaceum | 10 | -4.65 * ± 2.46 | 8.83 * ± 3.46 |
25 | -5.81 * ± 3.28 | 22.09 ** ± 2.40 | |
50 | -8.13 * ± 1.64 | 28.72 ** ± 3.79 | |
100 | -11.62 * ± 6.57 | 35.91 *** ± 3.02 | |
P. aeruginosa | 10 | 0.79 ± 0.62 | -9.19 ± 6.27 |
25 | 0.78 ± 0.44 | 15.47 * ± 3.33 | |
50 | -1.77 ± 0.47 | 22.61 * ± 4.89 | |
100 | -2.56 * ± 0.15 | 33.33 ** ± 3.83 |
* p<0.05, ** p<0.01, *** p<0.001; Catalase assay was done by monitoring disappearance of H 2O 2 at 240 nm; Hemoglobin concentration was measured at OD 540; DMSO in ‘vehicle control’ tube had no effect on catalase and haemolytic activity of any of the three bacteria; Chloramphenicol (0.5 μg/ml) enhanced catalase activity of C. violaceum by 11.23%*.± 0.01; Tetracycline (0.5 μg/ml) inhibited catalase activity of P. aeruginosa by 21.51%* ± 0.02.