Table 1.
Mass spectrometric identification and annotation of proteins isolated from dry seeds of maize that displayed differential accumulation in the hybrid DHM 117 compared to its parental inbred lines (BML 6 and BML 7) after separation on 2-D electrophoresis gels.
| Spot number | Expression patterna | Protein (species) accession numberb | Function classificationc | Mol. wt. gel/mol. (kDa) | pI gel/pI predictede | MASCOT scoref | Matched peptide g |
|---|---|---|---|---|---|---|---|
| 13 | F < H > M (AHP) | NAD(P)H-quinone oxidoreductase subunit K, chloroplastic (Glycine max) NDHK_SOYBN | Metabolism and energy | 21/24.1 | 5.7/6.82 | 25 | R.EIYENQMSSQR.E K.NSVISTTLNDLSNWSR.L |
| 18 | F < H > M (AHP) | Triosephosphate isomerase, cytosolic (Zea mays) gi|195605636 | Metabolism and energy | 28/27.2 | 5.7/5.53 | 303 | K.FFVGGNWK.C K.VIACVGETLEQR.E K.SQLRQEFHVAAQNCWVK.K K.VATPAQAQEVHASLRDWLK.T |
| 61 | F < H > M (AHP) | Maturase K (Thujaplicata) MATK_THUPL | Transcription and translation | 22.7/61.3 | 6.3/9.61 | 38 | K.GMDGWNHLR.S R.EDLHAIGHDHHLDR.S |
| 62 | F = H > M (HP) | Oil body-associated protein 2B/secreted protein (Zea mays) gi|226530017 | Defense and stress | 33.1/27.5 | 5.6/5.84 | 37 | R.LNQDVLQCAVYDSDKPSAR.L R.LIGVEYIVSDTIFEGLAPDEQR.L |
| 69 | F < H = M (HP) | Brittle 2 (Zea mays subsp. Mays) gi|23664291 | Metabolism and energy | 66/52.9 | 5.5/5.65 | 209 | R.FAPIYTQPR.H K.IYVLTQFNSASLNR.H K.AAANDSTYLNPQAHDSVLGIILGGGAGTR.L |
| 90 | F < H = M (D) | Globulin-1 S allele-like (Zea mays) gi|670440305 | Storage protein | 27/71.3 | 5.3/6.31 | 297 | K.GFETDVLR.L R.LGFGVKPEVVEAIK.S R.IYAIFTSEGINADDPSKPK.V |
| 105 | F = H > M (HP) | ATP synthase subunit beta, chloroplastic (Oltmannsiellopsis viridis) ATPB_OLTVI | Metabolism and energy | 15.1/51.6 | 6.2/4.70 | 41 | K.VINEDNLSESK.V R.MPSAVGYQPTLATEMGGLQER.I |
| 108 | F < H > M (AHP) | Glyoxalase family protein LOC100280456 (Zea mays) gi|226532762 | Defense and stress | 16/15.1 | 5.3/5.41 | 508 | K.AASFYDAAFGYTVR.R R.ETDELSGAVQLPDSSAAGR.G R.GSVEVCFAYADVDAAYKR.A R.KWAELESGATTIAFTPLHQR.E |
| 115 | F = H > M (HP) | Zein-beta (Zea mays) ZEB2_MAIZE | Storage protein | 20.4/20.2 | 6.8/7.48 | 82 | R.HQCSPAATPYGSPQCQALQQQCCHQIR.Q |
| 116 | F > H (Additive) | Myosin-9 (Arabidopsis thaliana) MYO9_ARATH | Cell cycle | 21.5/175.9 | 4.6/9.12 | 46 | K.FEIADIEPPPLIR.E R.MSQSFRGAPPGVNLAMINGAAGGGADTFR.Q |
| 122 | F > H (Additive) | NAD-dependent malic enzyme 62 kDa isoform, mitochondrial (Solanum tuberosum) MAOM_SOLTU | Metabolism and energy | 13.8/70.1 | 4.2/5.66 | 26 | R.LSSTLLKR.L K.IVVAGAGSAGIGVLNAARK.T |
| 137 | F < H (D) | Rab28 (Zea mays) gi|22460 | Defense and stress | 34.2/27.6 | 4.0/4.90 | 394 | R.IVTEFVAGQAVGQYLAR.D K.VTIGEALEATALAAGDAPVER.S R.LVADKPVESADALGVAGAENR.N |
| 154 | H < M (D) | Unknown (Zea mays) gi|224030527 | Storage protein | 23.3/65.3 | 5.1/6.39 | 478 | K.VFLAGADNVLQKLDR.V K.ALSFASKAEEVDEVLGSR.R R.LSPGTAFVVPAGHPFVAVASR.D K.ESGGHEEREQEEEEEREER.H R.SEEEEEESSEEQEEAGQGYHTIR.A R.RSEEEEEESSEEQEEAGQGYHTIR.A |
| 209 | H < M (Additive) | Cysteine protease ATG4 (Medicago truncatula) ATG4_MEDTR | Defense and stress | 66.3/54.0 | 6.7/6.69 | 40 | K.SSTEIVDNTQVPASSKAGSSDSK.F K.NEQGEQLLPMAIYVVSGDEDGER.G |
| 318 | F > H < M (BLP) | Nucleoredoxin 1 (Zea mays) gi|162459902 | Metabolism and energy | 76.5/64.0 | 4.8/4.8 | 100 | R.LEELQK.K K.VSGIPHLVILDAK.T K.MPWLAIPQGDIK.C R.CDECDFDLHPK.C K.MPWLAVPFSDSEGR.E K.MPWLAVPFSDSEGR.E R.GIPSLVAIGPTGQTVSR.D K.IRGIPSLVAIGPTGQTVSR.D K.SFEVVFASADRNEEAFNEYFAK.M |
a The expression pattern of differentially expressed protein spots in F1 hybrid (H) compared with parents (F - female, M - male) (AHP - above high parent, HP - high parent, LP - low parent, BLP - below low parent, D - different from additive, not belonging to any other class). b The accession number of each protein obtained via MASCOT software from the NCBInr and Swiss-Prot databases. c The function classification of each identified protein. d Molecular mass of proteins on gel and predicted molecular mass of proteins. e pI of proteins on gel and predicted pI of proteins. f Probability-based MASCOT score is defined as –10 × log (p), where p is the probability that the observed match is a random event. g Matching peptides unique to the identified proteins.