Table 2.
Mass spectrometric identification and annotation of proteins isolated from 24-h water-imbibed maize seeds that displayed differential accumulation in the hybrid DHM 117 compared to its parental inbreds (BML 6 and BML 7) after separation on 2-D electrophoresis gels.
| Spot number | Expression patterna | Protein (species) accession numberb | Function classificationc | Mol. wt. gel/mol. wt. predictedd (kDa) | pI gel/pI predictede | MASCOT scoref | Matched peptideg |
|---|---|---|---|---|---|---|---|
| 9 | F < H = M (HP) | Zein-beta (Zea mays) ZEB1_MAIZE | Storage protein | 17.8/19.9 | 7.3/8.15 | 221 | R.MMDVQSVAQQLQMMMQLER.A R.QQCCQQQMR.M |
| 26 | F = H < M (LP) | Vicilin-like embryo storage protein (Zea mays) gi|22284 | Storage protein | 22.8/66.6 | 5.3/6.23 | 354 | K.ALSFASKAEEVDEVLGSR.R R.SEEEEEESSEEQEEAGQGYHTIR.A R.RSEEEEEESSEEQEEAGQGYHTIR.A |
| 43 | F < H = M (HP) | Globulin-1 S allele-like (Zea mays) gi|670440305 | Storage protein | 31.6/71.3 | 5.4/6.31 | 287 | R.LGFGVKPEVVEAIK.S R.IYAIFTSEGINADDPSKPK.V R.VVAESEAGSVSAVDVADAAGTAYR.L |
| 58 | F = H < M (Additive) | Aldose reductase (Zea mays) gi|363543269 | Defense and stress | 37.8/35.9 | 6.9/6.47 | 146 | K.SGHTIPAVGLGTWR.A R.IKENIQVFGWEIPEEDFR.A R.IKENIQVFGWEIPEEDFR.A |
| 60 | F > H = M (LP) | Indole-3-acetaldehyde oxidase (Arabidopsis thaliana) ALDO2_ARATH | Hormone biosynthesis | 38/146.3 | 6.8/5.49 | 38 | K.LATHMEMIAAR.F K.VLYEAITLLGNVVVPEDGTSNPAYR.S |
| 64 | F > H = M (LP) | Type IV inositol polyphosphate 5-phosphatase 11 (Arabidopsis thaliana) IP5PB_ARATH | Defense and stress | 41/37.8 | 7.0/9.35 | 49 | K.TIEAVNSCSFSRK.A K.RDLTVWLGDLNYR.I |
| 136 | F > H = M (LP) | Chaperonin CPN60-1, mitochondrial (Zea mays) CH61_MAIZE | Defense and stress | 74.3/61.4 | 5.2/5.68 | 83 | K.APGFGENR.K R.NVVIEQSFGAPK.V |
| 154 | F = H < M (LP) | Sorbitol dehydrogenase homolog 1 (Zea mays) gi|926657619 | Metabolism and energy | 47.6/39.7 | 6.3/6.27 | 366 | R.EVDVVGVFR.Y R.VVVVDVDDHR.L R.VALEPGVSCWR.C K.AVGICGSDVHYLR.E K.IMPFKLPPVGPYDVR.V R.AGVGPETGVLVVGAGPIGLVSLLAAR.A |
| 172 | F > H < M (BLP) | Protein disulfide-isomerase (Zea mays) PDI_MAIZE | Metabolism and energy | 76/57.2 | 4.8/5.24 | 251 | K.FIDASTIPR.V K.NIQEYKGPR.E R.SDYDFGHTLHANHLPR.G K.LRSDYDFGHTLHANHLPR.G K.VDANEEKNRPLATKYEIQGFPTIK.I |
| 205 | F > H > M (D) | Globulin-1 S allele (Zea mays) GLB1_MAIZE | Storage protein | 65/65.4 | 7.1/6.63 | 304 | R.VAVLEANPR.S R.HGQDKGIIVR.A R.VLRPFDEVSR.L R.RSEEEEESSEEQEEVGQGYHTIR.A |
| 247 | F > H = M (LP) | Globulin-1 S allele (Zea mays) GLB1_MAIZE | Storage protein | 70/65.4 | 6.6/6.63 | 260 | R.RPYVFDRR.S R.VLRPFDEVSR.L K.IAYVPNGKGYAEIVCPHR.Q R.LSPGTAFVVPAGHPFVAVASR.D R.HASEGGHGPHWPLPPFGESR.G K.ILHTISVPGEFQFFFGPGGR.N |
| 279 | F < H = M (HP) | Oil body-associated protein 2A (Zea mays) gi|194693636 | Unknown | 30.5/27.8 | 7.1/6.90 | 286 | R.LIGVEYIVSR.K R.QVETHHYVSR.L K.GFAVDVVPHEMKR.H R.ADVEAPAEEHPGQADYWLR.H R.AARADVEAPAEEHPGQADYWLR.H |
| 319 | F = H < M (LP) | Glucose and ribitol dehydrogenase homolog (Oryza sativa subsp. Japonica) GRDH_ORYSJ | Metabolism and energy | 38.8/32.4 | 6.1/5.76 | 36 | R.GGAGAGGCSIINTSSINAYK.G |
| 321 | F > H < M (BLP) | Red chlorophyll catabolite reductase, chloroplastic (Arabidopsis thaliana) RCCR_ARATH | Defense and stress | 39.2/36.6 | 6.4/5.71 | 14 | K.EYYQDTALDSHR.Q |
| 377 | F < H (D) | Hydroxyproline-rich glycoprotein family protein (Zea mays) gi|226507242 | Metabolism and energy | 41.3/38.7 | 6.7/6.30 | 224 | K.LVPYNPGYQDESVLWTESR.D R.MVNNIYLNFDAFHGDKDHGGVR.D |
a The expression pattern of differentially expressed protein spots in F1 hybrid (H) compared with parents (F - female, M - male) (AHP - above high parent, HP - high parent, LP - low parent, BLP - below low parent, D - different from additive, not belonging to any other class). b The accession number of each protein obtained via MASCOT software from the NCBInr and Swiss-Prot databases. c The function classification of each identified protein. d Molecular mass of proteins on gel and predicted molecular mass of proteins. e pI of proteins on gel and predicted pI of proteins. f Probability-based MASCOT score is defined as –10 × log (p), where p is the probability that the observed match is a random event. g Matching peptides unique to the identified proteins.