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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Vet Comp Oncol. 2018 Aug 13;16(4):606–615. doi: 10.1111/vco.12428

FIGURE 3.

FIGURE 3

Pevonedistat increases apoptosis in canine DLBCL. A, Representative gating strategy for flow cytometric analysis of pevonedistat-treated cells for early apoptosis (Annexin V+, SYTOX red−), late apoptosis (annexin V+, SYTOX red+) and healthy cells (Annexin V−, SYTOX red−). B, Quantification of healthy, early apoptotic and late apoptotic CLBL-1. C, Western blot for cleaved caspase 3. CLBL-1 cells were treated with DMSO or pevonedistat (0.1–0.7 μM) for 6, 12 and 24 hours. Cell lysates were immunoblotted for cleaved caspase3 expression. β-actin was used as a loading control. D, Representative gating strategy for flow cytometric analysis of pevonedistat-treated primary canine DLBCL samples and quantification of early apoptotic cells. Early apoptotic cells were gated as Annexin V+ DAPI−, late apoptotic cells were gated as Annexin V+ DAPI+, and healthy cells were gated as Annexin V−DAPI−. **P < 0.01; ****P < 0.0001. Data was presented as mean ± SD