Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 10;39(3):496–512. doi: 10.1161/ATVBAHA.118.312315

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

PMC Copyright notice

Figure 5. — Ponatinib combined with rapamycin enhances apoptosis and inhibits cell migration in human umbilical vein endothelial cell (HUVEC)-TIE2-L914F. A, Flow cytometric analysis of apoptosis. HUVEC-TIE2-L914F cells were treated with control (dimethyl sulfoxide [DMSO]), 100 nmol/L ponatinib, 10 nmol/L rapamycin, or combination for 72 h. B, Quantification of % apoptotic cell populations. Data expressed as mean±SD, 1-way ANOVA for multiple comparisons (representative of 2 independent experiments, n=4). C, Representative images of cell cycle analysis. HUVEC-TIE2-L914F cells were treated with control (DMSO), 100 nmol/L ponatinib, 10 nmol/L rapamycin, or combination for 48 h. D, Quantification of % G0-G1 cell cycle cell population. Data expressed as mean±SD, 1-way ANOVA for multiple comparisons (representative of 2 independent experiments, n=4). E, Representative images of cells migrated through the 8 μm pore filter (Transwell migration assay). HUVEC-TIE2-L914F cells were treated with control (DMSO), 100 nmol/L ponatinib, 10 nmol/L rapamycin, or combination for 6 h and then stained with crystal violet. Scale bar: 100 μm. F, Quantification of migrated cells. Data expressed as mean±SD, 1-way ANOVA for multiple comparisons (n=3 independent experiments).