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. 2019 Feb 27;14(2):e0212732. doi: 10.1371/journal.pone.0212732

Table 1. Primers for real time PCR.

TARGET Sequence# Amplicon
Length
IL33 F: 5’-TGAGTCTCAACACCCCTCAA-3’ 169
MIP3α F: 5'-CTCCTGGCTGCTTTGATGTC-3' 151
MIP3β F: 5’-GTGCCTGCTGTAGTGTTCACC-3’ 133
GS F: 5’-GCCCCCTATCAAGGAACTT-3’ 173
Nestin F: 5’-GGCCATGACTCTGACCTCTC-3’ 190
GAPDH F: 5’-GTGGACCTCATGGCCTACAT-3’ 117

Amplification procedure: initial hot start activation (95°C/15min) followed by 39 cycles of Denaturation (94°C/10s) / Annealing (58°C/15s)/Extension (75°C/10s) and melting curve generation (55°C—95°C with one fluorescence reading every 0.5°C).

#Forward sequences are reported.