Table 1. Primers for real time PCR.
TARGET | Sequence# | Amplicon Length |
---|---|---|
IL33 | F: 5’-TGAGTCTCAACACCCCTCAA-3’ | 169 |
MIP3α | F: 5'-CTCCTGGCTGCTTTGATGTC-3' | 151 |
MIP3β | F: 5’-GTGCCTGCTGTAGTGTTCACC-3’ | 133 |
GS | F: 5’-GCCCCCTATCAAGGAACTT-3’ | 173 |
Nestin | F: 5’-GGCCATGACTCTGACCTCTC-3’ | 190 |
GAPDH | F: 5’-GTGGACCTCATGGCCTACAT-3’ | 117 |
Amplification procedure: initial hot start activation (95°C/15min) followed by 39 cycles of Denaturation (94°C/10s) / Annealing (58°C/15s)/Extension (75°C/10s) and melting curve generation (55°C—95°C with one fluorescence reading every 0.5°C).
#Forward sequences are reported.