Skip to main content
. 2019 Feb 14;13(2):e0007133. doi: 10.1371/journal.pntd.0007133

Table 1. Oligonucleotides used in this work.

Oligo No. Sequencea,b Purposedc Orientation
RBF919 ccgCTCGAGCCATGGCCACCATGGAGGACGCCAAGAACATCAA PpyRE9h Forward
RBF920 aaggaaaaaaGCGGCCGCTCAGATCTTGCCGCCCTTCTTGG PpyRE9h Reverse
RBF 630 CTTGTTTCAAGGACTTAGCCATG 5′integration Forward
RBF 637 TATTCGTTGTCAGATGGCGCAC 5′integration Reverse
RBF 644 CATGTGCAGCTCCTCCCTTTC 3′integration Forward
RBF 645 CCTTGTTACGACTTTTGCTTC 3′integration Reverse

aUnderlined sequence indicates restriction site.

bBold sequence indicates optimized translation initiation sequence.

cOrientation of primers: F, forward; R, reverse