Table 1. Oligonucleotides used in this work.
Oligo No. | Sequencea,b | Purposedc | Orientation |
---|---|---|---|
RBF919 | ccgCTCGAGCCATGGCCACCATGGAGGACGCCAAGAACATCAA | PpyRE9h | Forward |
RBF920 | aaggaaaaaaGCGGCCGCTCAGATCTTGCCGCCCTTCTTGG | PpyRE9h | Reverse |
RBF 630 | CTTGTTTCAAGGACTTAGCCATG | 5′integration | Forward |
RBF 637 | TATTCGTTGTCAGATGGCGCAC | 5′integration | Reverse |
RBF 644 | CATGTGCAGCTCCTCCCTTTC | 3′integration | Forward |
RBF 645 | CCTTGTTACGACTTTTGCTTC | 3′integration | Reverse |
aUnderlined sequence indicates restriction site.
bBold sequence indicates optimized translation initiation sequence.
cOrientation of primers: F, forward; R, reverse