Fig 1. The relative abundance of Bifidobacterium spp.

(A) and Lactobacillus spp. (B) in chyme from different sections of GIT in chickens supplemented in ovo with GOS prebiotic. GOS was delivered in ovo on day 12 of egg incubation. Samples of intestinal content were collected from 42-days-old chickens. Intestinal content was sampled from four distinct intestinal segments (duodenum, jejunum, ileum, and caecum) (n = 10). Bacteria quantification was done with qPCR based on bacterial DNA isolated from chyme. Statistical analysis was performed with two-way ANOVA with a Tukey HSD post hoc test. Significant differences found at P < 0.05.