Table 1. Overview regarding the used assays and observation time points.
| Assay | D0 | D1 | D7 | D14 | D21 | D42 |
|---|---|---|---|---|---|---|
| mCT | X | |||||
| ALP activity | X | X | X | X | X | |
| PrestoBlue | X | X | X | X | X | |
| dsDNA | X | X | X | X | X | |
| Live/dead-assay | X | X | ||||
| qPCR | N | X | X | X | X | |
| pH | X | X | X | X | X |
D: day of incubation, X: assay conducted. mCT-evaluation was performed before the incubation period started (D0). In order to obtain a baseline level and to provide normalization (N) for the subsequent assays, gene expression (qPCR)-analysis was performed after 24 hours of incubation (D1).