Table 1.

Primers, their sequences, and the PCR amplification conditions used in this study

Regions	Primer pairs	Sequence 5ʹ−3ʹ	Thermocycling conditions	Source
ITS	ITS‐5F	GGAAGTAAAAGTCGTAACAAGG	94°C 3 min; [35 cycles: 94°C 60 s, 53°C 90 s, 72°C 90 s]; 72°C 10 min	White, Bruns, Lee, & Taylor, (1990)
	ITS‐4R	CCTTATCATTTAGAGGAAGGAG
ITSa	ITS‐5F	GGAAGTAAAAGTCGTAACAAGG	94°C 3 min; [35 cycles: 94°C 60 s, 53°C 90 s, 72°C 90 s]; 72°C 10 min	Chen et al. (2010) and by Authors
	ITS‐300R	ATTCACACCAAGTATCGCAT
ITSb	ITS2‐2F	ATTCACACCAAGTATCGCAT
	ITS‐4R	CCTTATCATTTAGAGGAAGGAG
matK	3F‐KIM	CGTACAGTACTTTTGTGTTTACGAG	94°C 3 min; [35 cycles: 94°C 60 s, 52°C 60 s, 72°C 90 s]; 72°C 10 min	CBOL Plant Working Group (2009)
	1R‐KIM	ACCCAGTCCATCTGGAAATCTTGGTTC