Table 2.

Evaluation of three DNA barcoding regions

DNA regions	ITS	matK	ITS2
Percentage PCR success (%)	100	100	/
Sequencing using a single primer pair (%)	89.0	100	/
No. accessions/total	121	121	121
Sequence length (bp)	519–581	842–858	222–246
Aligned sequence length (bp)	680	883	275
No. variable sites (%)	258 (37.9)	219 (26.3)	123 (44.7)
No. informative sites (%)	194 (28.5)	167 (18.9)	94 (34.2)
No. indel (length in bp)	26 (1–22)	10 (1–12)	9 (1–22)
Intraspecific distance mean (range)	0.0103 (0–0.0317)	0.0016 (0–0.0062)	0.0159 (0–0.0579)
Interspecific distance mean (range)	0.0705 (0.0024–0.1185)	0.0474 (0–0.0878)	0.1026 (0.0014–0.1981)