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. 2018 Dec 1;8(3):225–235. doi: 10.1002/sctm.18-0098

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© 2018 The Authors. stem cells translational medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Differentiation potential of basal cell clones. Basal cells were recovered from bronchial tissue at the time of lung transplantation (non‐cystic fibrosis [CF] and CF), by brushing the bronchial epithelium (CF only), or brushing the nasal respiratory epithelium (CF only). Donor demographics are presented in Tables 1 and 2 and Supporting Information Table S1. (A): Cell density as a function of passage number was assessed by quantification of DAPI‐positive nuclei. (B): The frequency of MUC5B‐positive mucus cells was quantified as a function of passage. (C): The frequency of ciliated cells was assessed by quantification of cells expressing multiple ACT positive apical cilia. Data are presented as the mean ± SD, n = 22 clones. Symbols represent data from different clones. Abbreviation: ACT, acetylated tubulin.