Figure 1.

Experimental method and paradigm. (A): Vitrification process. (B): Control and patient cell lines used for all experiments. (C and D): hiPSCs (C) and smNPCs (D) were cryopreserved via slow‐rate freezing in suspension and adherent vitrification in TWIST substrate. Experiments were performed for unfrozen cells and cryopreserved cells 1 day (d1) and 4 days (d4) after rapid thawing using immunocytochemistry, fluorescence‐activated cell sorting analysis, RNA‐sequencing, and scanning electron microscopy. Abbreviations: C, control; CPA, cryoprotective agents; hiPSCs, human‐induced pluripotent stem cells; PD, Parkinson's disease; smNPCs, small molecule neural precursor cells.