Figure 3.

Treatment with TMP778 inhibits the expression of both RORγt and T-bet. (A) Flow cytometric analysis of intracellular expression of T-bet and RORγt by lymphoid cells collected from eyes of mice on day 14 post-immunization. Representative flow plots displaying the frequency of T-bet⁺ Th1 cells or RORγt⁺ Th17 cells in the eyes. Data are shown as +- SEM (n=4) and are from one experiment representative of four independent experiments. ** p<0.01; unpaired t test. (B) Quantitative PCR analysis of TMP778 treatment effect on transcript expression of transcription factor genes. Spleen cells of TMP778 treated mice and of their vehicle-treated controls were processed as detailed elsewhere [23]. Data are shown as +- SEM (n=3) and are pooled from three independent experiments. Please note that T-bet transcript expression is suppressed in the TMP778-treated mice, similarly to RORγt transcript. * p <0.05; two-tailed t test.