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. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Methods. 2019 Jan 6;155:77–87. doi: 10.1016/j.ymeth.2019.01.003

Fig. 3. Pumilio enhances decay of a reporter mRNA bearing Pum Response Elements (PREs) in d.mel-2 (S2) insect cells.

Fig. 3.

A) Northern blot of a transcription shut-off experiment. Plasmids bearing either wild type (WT) or RNA binding defective (mut R7) Pum were expressed in d.mel-2 cells, along with the Nanoluc (Nluc) 3xPRE reporter. Nluc mRNA was detected with a probe to the open reading frame of Nluc. mRNA from untransfected (UT) cells served as a negative control to demonstrate specificity of the probe. B) Decay curves from transcription shut-off experiments,generated by Graphpad Prism. The fraction of mRNA remaining was plotted versus time (hours).