Skip to main content
. Author manuscript; available in PMC: 2020 Feb 15.
Published in final edited form as: Methods. 2019 Jan 6;155:77–87. doi: 10.1016/j.ymeth.2019.01.003

Figure 4: Depletion of the Pop2 deadenylase slows decay of cyclin B mRNA in d.mel-2 (S2) insect cells.

Figure 4:

A) Northern blot of transcription shut-off experiment to measure endogenous cyclin b mRNA decay in d.mel-2 cells treated with dsRNA to either a non-targeting control (NTC) or the Pop2 deadenylase. B) Decay curves from a transcription shut-off experiment, generated by Graphpad Prism. The fraction of mRNA remaining was plotted versus time (hours).