Figure 2.

Intra-amniotic Ureaplasma parvum infection decreased fetal regulatory T cell (Treg) proportion. Cell suspensions from the thymus, spleen and, blood mononuclear cells were analyzed by flow cytometry. Box-and-whisker plots show frequency of Tregs (defined as forkhead box [FOX] P3⁺) within total CD4⁺CD3⁺ T cells in cord blood mononuclear cells (A), spleen (B), and total CD4 single-positive thymocytes (C), defined as CD34⁻CD7⁺CD3⁺CD4⁺CD8a⁻ cells. Boxes represents 25th–75th percentiles; horizontal line, medians; and whiskers, 5th and 95th percentile values (n = 5–6 per group). *P < .05 vs controls (Mann–Whitney U test).