Fig. 3. Ephrin-B3 is down-regulated in the excited DG region under voluntary running.
(A) 3D reconstruction of confocal images of rNSCs and GCs in Nestin-GFP mice that were injected with AAV-CaMKII-mCherry. The confocal Z stack images were simulated to 3D images with LAS X software. The arrowheads indicate the direct contact areas of rNSCs and GCs. (B) Results of GO Enrichment analysis on the set of 1802 DEGs. The length of each bar indicates the log10 (P value), and the vertical axis shows significantly enriched terms. (C) The selected nine genes with differential expression are shown in the heat map. (D) mRNA from DG excited by the chemogenetic approach was used to detect differential expression of genes selected from previous sequencing data after running (28). (E) The expression of ephrin-B3 in DG tissue from control and hM3Dq mice was detected by Western blot. (F) The expression of ephrin-B3 in DG tissue from control and hM4Di mice under voluntary running treatment was detected by Western blot. Results are presented as means ± SEM. *P < 0.05; ***P < 0.001; ****P < 0.0001.