Table 2.

Central composite design arrangement for extraction of polyphenols and carotenoids from Centella asiatica

Run order	Ethanol%	Temperature (°C)	Time (min)	Polyphenols mg/g DW	Carotenoids mg/g DW
1	30	60	90	4.13	0.71
2	123.9	45	60	1.43	2.44
3	65	45	9.5	3.06	1.93
4	30	30	90	3.64	0.73
5	65	45	60	3.43	2.17
6	65	45	60	3.99	2.37
7	65	45	60	2.27	3.64
8	65	19.8	60	4.31	2.47
9	100	30	30	1.54	2.45
10	65	45	110.5	3.72	2.75
11	65	45	60	3.24	2.76
12	30	60	30	3.57	1.11
13	30	30	30	3.31	0.98
14	100	30	90	1.61	2.57
15	65	45	60	3.38	2.5
16	65	70.2	60	4.09	2.71
17	6.1	45	60	2.98	0.89
18	100	60	90	2.24	3.47
19	100	60	30	1.65	2.61
20	65	45	60	3.42	2.23