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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Hepatology. 2019 Feb 13;69(3):1004–1019. doi: 10.1002/hep.30266

Figure 1. lncRNA-IFI6 regulation of IFI6 expression and HCV infection.

Figure 1.

JFH1 HCV was inoculated in to cells for 48 hrs. At 24 hrs post infection, human IFNα was added at a final concentration of 30 IU/ mL. Total RNA or protein lysates were harvested at 48 hrs post infection. (A) lncRNA-IFI6 gRNA decreased lncRNA-IFI6 levels in Huh7.5.1 or JFH1 cells compared to Neg gRNA with or without IFNα, respectively. (B) lncRNA-IFI6 gRNA reduced HCV RNA levels in JFH1-infected Huh7.5.1 cells compared to Neg gRNA. IFNα plus lncRNA-IFI6 gRNA further decreased HCV RNA levels. (C) lncRNA-IFI6 gRNA significantly increased IFI6 mRNA expression in Huh7.5.1 and JFH1 cells compared to Neg gRNA with or without IFNα, respectively. (D) lncRNA-IFI6 gRNA increased IFI6 protein and reduced HCV core levels in Huh7.5.1 or JFH1 cells compared to Neg gRNA with or without IFNα, respectively. (E) lncRNA-IFI6 gRNA reduced HCV RNA levels in PHHs or JFH1-infected PHH cells compared to Neg gRNA. IFNα and lncRNA-IFI6 gRNA further decreased HCV RNA levels. (F) lncRNA-IFI6 gRNA significantly increased IFI6 mRNA expression in PHH and JFH1-infected PHH cells compared to Neg gRNA with or without IFNα, respectively.