A, Activity of microvesicles-associated tissue factor
(MV-TF) in the plasma after preincubation with IgG or TF 1H1 antibody
(n=6/group). Data are presented by subtracting the amount of FXa generated in
the presence of TF 1H1 antibody from the amount of total FXa generated in the
presence of IgG. B, F3 mRNA level of carotid
arteries from WT and Klf11 KO mice. The mRNA level was
normalized by 18S and is presented relative to the WT group set
as 1 (n=4/group). C, The aortic TF activity was measured and
presented as in A, after preincubation with IgG or TF 1H1 antibody
and normalized to the total protein quantity (n=6/group).
**P<0.01 using unpaired Student t-test
(A-C). D, Expression of TF (Alexa
647, displayed in green) and α-SMA (α-smooth muscle actin, Alexa
568, displayed in red) in mouse aorta at basal level or 4 hours after LPS (30
µg/kg) tail vein injection was visualized by immunofluorescence staining.
Respective IgG staining was used as negative control. Scale bars=50 µm.
Quantification was performed from 4 mice, randomly selecting 3 different medial
regions from each specimen and dividing the TF immunofluorescence intensity by
medial area (indicated by α-SMA positive cells). Data are presented
relative to the basal level of WT group set as 1.
**P<0.01 using two-way ANOVA followed by Bonferroni
test.