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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Mar;39(3):402–412. doi: 10.1161/ATVBAHA.118.311612

Figure 3. KLF11 (Krüppel-like Factor 11) overexpression rescues the TF (tissue factor) upregulation in KLF11-deficient MASMCs (mouse aortic smooth muscle cells).

Figure 3.

MASMCs were isolated from male (A-D) and female (E-H) WT or Klf11 KO mice. A and E, Klf11 mRNA level of MASMCs from WT and Klf11 KO mice. The mRNA level was normalized by 18S and is presented relative to the WT group set as 1 (n=4/group). **P<0.01 using unpaired Student t-test. B-H, MASMCs isolated from WT or Klf11 KO mice were infected with Ad-LacZ or Ad-KLF11 (50 MOI). B and F, F3 mRNA level of MASMCs was normalized by 18S and is presented relative to the WT infected with Ad-LacZ group set as 1 (n=4/group). C and G, Representative western blot of TF protein level. D and H, Band density from 4 independent western blots was quantitatively analyzed and normalized against β-actin. The WT infected with Ad-LacZ group was set as 1. **P<0.01 or NS, no significance using two-way ANOVA followed by Bonferroni test (B, D, F, H).