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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Mar;39(3):402–412. doi: 10.1161/ATVBAHA.118.311612

Figure 4. TF (Tissue factor) expression is increased upon KLF11 (Krüppel-like Factor 11) knockdown in HASMCs (human aortic smooth muscle cells).

Figure 4.

HASMCs were transfected with si-Control or si-KLF11 (40 nM) and 24 hours later serum starved with 0.5% FBS (fetal bovine serum) for 48 hours. Three days after transfection, HASMCs were exposed to thrombin (3.24 µg/ml) (B-D) or 10% FBS (E-G) for 4 hours. A, The knockdown efficiency of KLF11 was determined by real-time quantitative PCR and western blot. The mRNA level was normalized by GAPDH and is presented relative to HASMCs transfected with si-Control group set as 1 (n=4/group). **P<0.01 using unpaired Student’s t-test. B and E, F3 mRNA level of HASMCs was normalized by GAPDH and is presented relative to HASMCs transfected with si-Control group set as 1 (n=4/group). C and F, Representative western blot showing the protein level of TF. D and G, Band density from 4 independent western blots was quantitatively analyzed and normalized against GAPDH. HASMCs transfected with si-Control group was set as 1. *P<0.05, **P<0.01 using two-way ANOVA followed by Bonferroni test (B, D, E, G).