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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Mar;39(3):402–412. doi: 10.1161/ATVBAHA.118.311612

Figure 5. TF (Tissue factor) expression is decreased upon KLF11 (Krüppel-like Factor 11) overexpression in HASMCs (human aortic smooth muscle cells).

Figure 5.

HASMCs were infected with Ad-LacZ or Ad-KLF11 (50 MOI). Twelve hours after infection, HASMCs were serum starved with 0.5% FBS (fetal bovine serum) for 48 hours and then stimulated to thrombin (3.24 µg/ml) (B-D) or 10% FBS (E-G) for 4 hours. A, The overexpression of KLF11 was determined by western blot. B and E, F3 mRNA level of HASMCs from each group was normalized by GAPDH and is presented relative to HASMCs infected with Ad-LacZ group set as 1 (n=4/group). C and F, Representative western blot showed the protein level of TF. D and G, Band density from 4 independent western blots was quantitatively analyzed and normalized against GAPDH. HASMCs infected with Ad-LacZ group was set as 1. **P<0.01 using two-way ANOVA followed by Bonferroni test (B, D, E, G).