Figure 9.

Crosslinking modulation of vascularized adipose tissue graft in cell-laden collagen hydrogels. 0.6 % Collagen pre-polymer solutions with ECFCs and MSCs in the absence of any exogenous cytokines were subcutaneously injected into nude mice and formed cell-laden collagen hydrogels. Constructs were evaluated after 1 month in vivo. (a) The macroscopic view (inset, scale bar 5 mm) and representative H&E-stained section of entire cell-laden collagen constructs are shown (scale bar 1 mm). (b-d) High magnification of selected regions show some murine adipocytes (human-vimentin-negative) with murine vessels (human CD31-negative, blue arrows) carrying erythrocytes.