Figure 2.

Cocaine-mediated initiation of autophagy and ER stress in human primary pericytes (HBVP). Representative western blots showing time-dependent upregulation of BiP (A), ATF 6 (B), IRE 1α (C), p-PERK/ PERK (D), p-EIF/ EIF (E), BECN1 (F), LC3B-II (G) and p62 (H) in HBVP exposed to cocaine (10 μM) for the indicated time points. β-actin was used as a loading control for all experiments. Data are presented as mean ± SEM; the mean is derived from 6 independent experiments (n = 6). One-way ANOVA followed by Bonferroni post hoc test was used to determine the statistical significance between multiple groups: *, P < 0.05 vs. control.