Cocaine-mediated ER stress-dysregulated autophagy mediated inflammation
involves σ−1Rin HBVP. Representative western blots showing the
protein levels of BiP (A), ATF 6 (B), IRE 1α (C), p-PERK/ PERK (D),
p-EIF/ EIF (E), BECN1 (F), LC3B-II (G) and p62 (H) in HBVP pretreated with 10
μM BD 1047 for 1 h followed by 10 μM cocaine exposure for 12h. (I)
RT-qPCR showing relative expression of proinflammatory cytokines such as
TNF-α, IL 1β and IL 6 mRNA in HBVP pretreated with 10 μM BD
1047 for 1 h followed by 10 μM cocaine exposure for 6 h. Data are
presented as mean ± SEM; the mean is derived from 6 independent
experiments (n = 6). Abbreviation: Coc: Cocaine. One-way ANOVA followed by
Bonferroni post hoc test was used to determine the statistical significance: *,
P<0.05 vs. control; #, P<0.05 vs. cocaine.