Figure 6.

Aptamer binding studies. Above: Secondary structures of aptamer candidates 42, B2–1, 1 and 12 derived from common LocARNA analysis. Below: Radioactively labeled aptamer RNA was folded and immobilized on azoCm derivatized columns using the same conditions as during SELEX. Four individual elution steps with 50 μM azoCm (in the trans, cis and cis-trans switched conformation) or buffer were performed. The percentage of eluted RNA versus total RNA was calculated. Each aptamers azoCm trans value was normalized to 100%. Buffer elution values are marked with a line in each graph.