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. 2018 Dec 7;47(4):1692–1705. doi: 10.1093/nar/gky1230

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 8. — FOXO1 protein levels were decreased by G9a in colon cancer patients tissue. (A) Formalin-fixed tissue array slides were used in immunohistochemistry experiments. Tissue array slides from colon cancer and normal tissues were used for immuno-histochemical 3,3-diaminobenzidine (DAB) staining of FOXO1 or G9a. (B–D) Colon cancer patients tissue were lysed and immunoblotted with anti-FOXO1 anti-G9a and anti-β-actin antibodies. (B) TNM grade 1, (C) TNM grade 2 and (D) TNM grade 3. FOXO1 and G9a protein levels were normalized by β-actin. The dotted line indicates the degree of expression of normal tissue.