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Cartoon of a Mouse Embryonic Fibroblast (MEF). (A) Cell cross-section and confocal slices at cell's bottom, mid-height, and top. (B) Mid plane cross-section. The average fluorescence intensity emitted from the indicated 5 × 5 pixel rectangular regions next to the nuclear membrane was used to determine the ratio between the nuclear and cytoplasmic fluorescence intensity (FIR) and in the model we use to estimate reaction rate constants.
