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. 2018 Nov 28;47(4):2002–2010. doi: 10.1093/nar/gky1208

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Cytoplasmic tRNA shuttling between nucleus and cytoplasm when the cell media is changed during observation. (A) Cells are deprived of nutrition by replacing fresh media with PBS after 30 min of observation, resulting in retrograde nuclear accumulation (N = 27). (B) The cell is replenished with fresh media after 30 min in PBS (0% nutrition), resulting in tRNA export from the nucleus to the cytoplasm (N = 6).