Table 1.
Rate constants for different nutritional media (the numbers in parenthesis correspond to standard error)
| Nutrition | k in (min−1) | k out (min−1) | FIR∞ = (kin/kout) | τ (min) | R 2 *** | # of cells analyzed |
|---|---|---|---|---|---|---|
| 100% nutrition* | 0.30 (0.04) | 0.32 (0.04) | 1.0 (0.1) | 2.40 (0.3) | 0.58 | 20 |
| 50% nutrition* | 0.16 (0.03) | 0.10 (0.02) | 1.6 (0.2) | 8.8 (2.2) | 0.97 | 4 |
| 25% nutrition* | 0.18 (0.04) | 0.08 (0.03) | 2.2 (0.2) | 12.6 (2.7) | 0.97 | 6 |
| No nutrition* | 0.18 (0.04) | 0.06 (0.02) | 3.0 (0.5) | 14.3(2.0) | 0.96 | 20 |
| 100% nutrition + 2 mM puromycin | n.d. | n.d. | 0.81 (0.1) | 1.06 (0.1)** | 0.31 | 11 |
| no nutrition + 2 mM puromycin | n.d. | n.d. | 0.9 (0.06) | 1.13 (0.1)** | 0.28 | 5 |
| 100% nutrition + 1 min nocodazole | n.d. | n.d. | 0.87 | 9.3 (2.3)** | 0.77 | 11 |
| 100% nutrition + 1 h nocodazole | n.d. | n.d. | 1.16 | 10.5 (1.5)** | 0.94 | 7 |
| 100% nutrition + 2 h nocodazole | n.d. | n.d. | 1.22 | 10 (1.5)** | 0.93 | 6 |
| 100% nutrition + 3 h nocodazole | n.d. | n.d. | 1.45 | 6.8 (1.1)** | 0.94 | 6 |
| 100% nutrition + 4 h nocodazole | n.d. | n.d. | 1.4 | 5.5 (0.9)** | 0.95 | 6 |
* Reaction rate constants determined by fitting data to Equation (1).
** Time constants determined by fitting data to equation SE7.
*** Coefficient of determination [42]
n.d. – not determined. Attempts to fit the data to Equation (1) in the presence of either puromycin or nocodazole failed to converge. The reaction rate constants were determined with equation SI–SE8.