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. 2018 Dec 4;47(4):2011–2028. doi: 10.1093/nar/gky1209

Figure 5.

Figure 5.

RPS3 up-regulates SIRT1 expression by stabilizing SIRT1 mRNA. (A) RPS3 overexpression prolonged SIRT1 mRNA half-life. (B) RPS3 knockdown shortened SIRT1 mRNA half-life. (C) RPS3 overexpression increased the mRNA and protein levels of SIRT1. (D) RPS3 knockdown decreased the mRNA and protein levels of SIRT1. (E–I) RPS3 regulates SIRT1 by interacting with SIRT1 mRNA. PGL3-derived luciferase reporter vectors bearing the 5′UTR, CDS, 3′UTR and different fragments of the SIRT1 3′UTR were co-transfected with Renilla luciferase vectors into 293T cells. Luciferase activities normalized against Renilla luciferase activities were measured by the Dual-Luciferase Reporter Assay System. *P < 0.05, **P < 0.01 and ***P < 0.001.