Table 1.
Data collection and refinement statistics for T. brucei TbRGG2 RRM and TbRGG2 RRM-RNA complex
| TbRGG2 RRM(L209M) | WT TbRGG2 RRM | WT TbRGG2 RRM-U4 RNA | |
|---|---|---|---|
| Data collection | |||
| Pdb code | 6E4N | 6E4O | 6E4P |
| Space group | R3 | P41212 | P21 |
| Cell dimensions | |||
| a, b, c (Å) | 59.8, 59.8, 57.5 | 72.1,72.1, 118.7 | 49.1,126.3, 49.2 |
| α, β, γ (°) | 90.0, 90.0, 120.0 | 90.0, 90.0, 90.0 | 90.0, 118.6, 90.0 |
| Resolution (Å) | 19.26–1.80 | 38.64–1.80 | 35.66–1.95 |
| Total reflections (#) | 23 430 | 74 490 | 146 384 |
| Unique reflections (#) | 7045 | 28 448 | 38 345 |
| R sym | 0.070 (0.249)* | 0.050 (0.212) | 0.066 (0.351) |
| R pim | 0.044 (0.161) | 0.035 (0.208) | 0.040 (0.280) |
| CC(1/2) | 0.994 (0.960) | 0.997 (0.977) | 0.998 (0.990) |
| Completeness (%) | 96.0 (91.3) | 96.0 (80.3) | 99.8 (99.0) |
| Redundancy | 3.2 (1.7) | 2.6 (1.4) | 3.8 (3.5) |
| I/σI | 11.3 (4.2) | 12.5 (2.3) | 17.8 (2.5) |
| Resolution (Å) | 19.26–1.80 | 38.64–1.80 | 35.66–1.95 |
| R work/Rfree (%) | 15.1/18.7 | 16.5/19.7 | 18.1/22.9 |
| R.M.S. deviations | |||
| Bond lengths (Å) | 0.004 | 0.006 | 0.005 |
| Bond angles (°) | 0.763 | 0.890 | 0.848 |
| Ramachandran analyses | |||
| Most favored (%) | 100.0 | 99.4 | 99.7 |
| Disallowed (%) | 0.0 | 0.0 | 0.0 |
*Values in parentheses are for highest-resolution shell.