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. 2018 Dec 12;47(4):2056–2074. doi: 10.1093/nar/gky1241

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Western blot analysis of OXPHOS subunits. (A) Twenty micrograms of total cellular proteins from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with antibody cocktail specific for subunits of each OXPHOS complex and with β-actin as a loading control. (B) Quantification of the levels of ATP5A, UQCRC2, SDHB, CO₂ and NDUFB8 in mutant and control cell lines were determined as described elsewhere (52). Graph details and symbols are explained in the legend to Figure 3.